Format

Send to

Choose Destination
Pharmacol Rep. 2014 Feb;66(1):179-83. doi: 10.1016/j.pharep.2013.06.012. Epub 2014 Feb 1.

Oral dosing in adult zebrafish: proof-of-concept using pharmacokinetics and pharmacological evaluation of carbamazepine.

Author information

1
Dr Reddy's Institute of Life Sciences, University of Hyderabad Campus, Gachibowli, Hyderabad, India; Zephase Therapeutics (An Incubated Company at the Dr Reddy's Institute of Life Sciences), University of Hyderabad Campus, Gachibowli, Hyderabad, India. Electronic address: pushkark@drils.org.
2
Dr Reddy's Institute of Life Sciences, University of Hyderabad Campus, Gachibowli, Hyderabad, India; Zephase Therapeutics (An Incubated Company at the Dr Reddy's Institute of Life Sciences), University of Hyderabad Campus, Gachibowli, Hyderabad, India.
3
Dr Reddy's Institute of Life Sciences, University of Hyderabad Campus, Gachibowli, Hyderabad, India; Cosmic Discoveries, (An Incubated Company at the Dr Reddy's Institute of Life Sciences), University of Hyderabad Campus, Gachibowli, Hyderabad, India.
4
Dr Reddy's Institute of Life Sciences, University of Hyderabad Campus, Gachibowli, Hyderabad, India; Cosmic Discoveries, (An Incubated Company at the Dr Reddy's Institute of Life Sciences), University of Hyderabad Campus, Gachibowli, Hyderabad, India. Electronic address: soruganti@drils.org.
5
Dr Reddy's Institute of Life Sciences, University of Hyderabad Campus, Gachibowli, Hyderabad, India; Zephase Therapeutics (An Incubated Company at the Dr Reddy's Institute of Life Sciences), University of Hyderabad Campus, Gachibowli, Hyderabad, India. Electronic address: kiranamc@drils.org.

Abstract

BACKGROUND AND METHODS:

We describe a method for obtaining pharmacokinetics (PK) and pharmacology data from adult zebrafish in terms of mg/kg using a novel method of oral administration. Using carbamazepine (CBZ) as a test drug, we employed dried blood spot (DBS) cards to enable drug quantification for PK; and we evaluated the pharmacological anxiolytic effect using novel tank test.

RESULTS:

The PK study confirmed the presence of CBZ in both blood and brain and the behavioural study showed dose dependent anxiolytic effect. The reproducibility of oral dosing was confirmed by the fact that the results obtained in both the experiments had negligible errors.

CONCLUSIONS:

This report enables a novel approach for optimizing the utility of zebrafish in drug discovery and drug delivery research.

KEYWORDS:

Drug delivery; Drug discovery; Oral administration; Pharmacokinetics; Zebrafish

PMID:
24905326
DOI:
10.1016/j.pharep.2013.06.012
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center