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J Vet Diagn Invest. 2014 Jul;26(4):585-587. doi: 10.1177/1040638714535601.

Isolation of Moraxella bovoculi from racehorses with keratoconjunctivitis.

Liu H1,2,3,4,5, Yan J1,2,3,4,5, Wang Y1,2,3,4,5, Yan Q1,2,3,4,5, Zhao L1,2,3,4,5, Yan R1,2,3,4,5, He H1,2,3,4,5.

Author information

1
National Research Center for Wildlife-borne Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing (Liu, J Yan, Q Yan, He), P.R. China.
2
University of Chinese Academy of Sciences, Beijing (Liu), P.R. China.
3
General Station of Animal Husbandry of Beijing (Wang), P.R. China.
4
Henan Center for Animal Disease Control & Prevention, Animal Husbandry Bureau of Henan Province, Zhengzhou (R Yan), P.R. China.
5
Zhengzhou Zhongdao Biotechnology Co. Ltd. (Zhao), P.R. China.

Abstract

Moraxella bovoculi was isolated and identified in ocular fluid samples collected from 9 racehorses with infectious keratoconjunctivitis in China in 2013. All 9 M. bovoculi isolates were hemolytic, Gram-negative diplococci that were phenylalanine deaminase positive. The sequence of the 16S ribosomal DNA (rDNA) gene of the isolates matched the 16S rDNA sequence of M. bovoculi. Amplification of the 16S-23S intergenic spacer region followed by AfaI digestion produced a 600-base pair product, a result characteristic of M. bovoculi isolates. The phylogenetic analysis based on the 16S rDNA sequence confirmed the strain isolated in the current study had genetic homology with M. bovoculi.

KEYWORDS:

Keratoconjunctivitis; Moraxella bovoculi; racehorses

PMID:
24903634
DOI:
10.1177/1040638714535601
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