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Int J Syst Evol Microbiol. 2014 Sep;64(Pt 9):2944-8. doi: 10.1099/ijs.0.057778-0. Epub 2014 Jun 4.

Campylobacter fetus subsp. testudinum subsp. nov., isolated from humans and reptiles.

Author information

1
Biotechnology Core Facility Branch, Division of Scientific Resources, National Center for Emerging and Zoonotic Infectious Diseases, CDC, Atlanta, GA, USA chf3@cdc.gov.
2
Department of Medicine, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA.
3
Biotechnology Core Facility Branch, Division of Scientific Resources, National Center for Emerging and Zoonotic Infectious Diseases, CDC, Atlanta, GA, USA.
4
Massachusetts Department of Public Health, Jamaica Plain, MA, USA.
5
Public Health England, London, UK.
6
Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands WHO Collaborating Center for Campylobacter/OIE Reference Laboratory for Campylobacteriosis, Utrecht, The Netherlands.
7
WHO Collaborating Center for Campylobacter/OIE Reference Laboratory for Campylobacteriosis, Utrecht, The Netherlands Central Veterinary Institute of Wageningen UR, Lelystad, The Netherlands.
8
USDA, ARS, WRRC, Produce Safety and Microbiology Research Unit, Albany, CA, USA.
9
Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands.
10
Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands WHO Collaborating Center for Campylobacter/OIE Reference Laboratory for Campylobacteriosis, Utrecht, The Netherlands Central Veterinary Institute of Wageningen UR, Lelystad, The Netherlands.

Abstract

A polyphasic study was undertaken to determine the taxonomic position of 13 Campylobacter fetus-like strains from humans (n = 8) and reptiles (n = 5). The results of matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) MS and genomic data from sap analysis, 16S rRNA gene and hsp60 sequence comparison, pulsed-field gel electrophoresis, amplified fragment length polymorphism analysis, DNA-DNA hybridization and whole genome sequencing demonstrated that these strains are closely related to C. fetus but clearly differentiated from recognized subspecies of C. fetus. Therefore, this unique cluster of 13 strains represents a novel subspecies within the species C. fetus, for which the name Campylobacter fetus subsp. testudinum subsp. nov. is proposed, with strain 03-427(T) ( = ATCC BAA-2539(T) = LMG 27499(T)) as the type strain. Although this novel taxon could not be differentiated from C. fetus subsp. fetus and C. fetus subsp. venerealis using conventional phenotypic tests, MALDI-TOF MS revealed the presence of multiple phenotypic biomarkers which distinguish Campylobacter fetus subsp. testudinum subsp. nov. from recognized subspecies of C. fetus.

PMID:
24899653
DOI:
10.1099/ijs.0.057778-0
[Indexed for MEDLINE]

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