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Curr Protoc Toxicol. 2014 Feb 20;59:12.21.1-24. doi: 10.1002/0471140856.tx1221s59.

Comparative human and rat "neurosphere assay" for developmental neurotoxicity testing.

Author information

1
IUF-Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany; Both authors contributed equally to this unit.

Abstract

The developing nervous system is highly vulnerable to the adverse effects of chemical agents. Currently, there is an increasing need for testing and regulating chemical compounds in general use and, due to the lack of available data, to identify those which are developmental neurotoxicants. In this context, alternative testing strategies are needed in order to allow fast and cost-efficient screening and to reduce the number of animal experiments usually required. In this unit we present an in vitro three-dimensional model for developmental neurotoxicity screening based on human and rat neural progenitor cells. This model enables the detection of disturbances in basic processes of brain development, such as proliferation, migration, differentiation and apoptosis, and allows the distinction of these specific disturbances from general cytotoxicity. Furthermore, the comparison of human and rat data provides useful insights into species differences for toxicodynamics of compounds contributing to human risk assessment of developmental neurotoxicants.

KEYWORDS:

brain development; developmental neurotoxicity; in vitro; neural progenitor cell; species differences

PMID:
24898107
DOI:
10.1002/0471140856.tx1221s59
[Indexed for MEDLINE]

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