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Biomed Res Int. 2014;2014:959206. doi: 10.1155/2014/959206. Epub 2014 May 5.

Identification of virulence factors genes in Escherichia coli isolates from women with urinary tract infection in Mexico.

Author information

1
Department of Ecology of Pathogen Agents, Hospital General "Dr. Manuel Gea González", 14080 Tlalpan, DF, Mexico.
2
Department of Molecular Biology and Histocompatibility, Hospital General "Dr. Manuel Gea González", 14080 Tlalpan, DF, Mexico.
3
Institute of Cell Physiology, Universidad Nacional Autónoma de México, 04510 Coyoacán, DF, Mexico.
4
Department of Public Health, Universidad Nacional Autónoma de México, 04510 Coyoacán, DF, Mexico.
5
Clinical Laboratory, Hospital General "Dr. Manuel Gea González", 14080 Tlalpan, DF, Mexico.
6
Department of Infectology, Hospital Infantil de México "Federico Gómez", 06720 Cuauhtémoc, DF, Mexico.

Abstract

E coli isolates (108) from Mexican women, clinically diagnosed with urinary tract infection, were screened to identify virulence genes, phylogenetic groups, and antibiotic resistance. Isolates were identified by MicroScan4 system; additionally, the minimum inhibitory concentration (MIC) was assessed. The phylogenetic groups and 16 virulence genes encoding adhesins, toxins, siderophores, lipopolysaccharide (LPS), and invasins were identified by PCR. Phylogenetic groups distribution was as follows: B1 9.3%, A 30.6%, B2 55.6%, and D 4.6%. Virulence genes prevalence was ecp 98.1%, fimH 86.1%, traT 77.8%, sfa/focDE 74.1%, papC 62%, iutA 48.1%, fyuA 44.4%, focG 2.8%, sfaS 1.9%, hlyA 7.4%, cnf-1 6.5%, cdt-B 0.9%, cvaC 2.8%, ibeA 2.8%, and rfc 0.9%. Regarding antimicrobial resistance it was above 50% to ampicillin/sulbactam, ampicillin, piperacillin, trimethoprim/sulfamethoxazole, ciprofloxacin, and levofloxacin. Uropathogenic E. coli clustered mainly in the pathogenic phylogenetic group B2. The isolates showed a high presence of siderophores and adhesion genes and a low presence of genes encoding toxins. The high frequency of papC gene suggests that these isolates have the ability to colonize the kidneys. High resistance to drugs considered as first choice treatment such as trimethoprim/sulfamethoxazole and fluoroquinolones was consistently observed.

PMID:
24895634
PMCID:
PMC4026957
DOI:
10.1155/2014/959206
[Indexed for MEDLINE]
Free PMC Article

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