Format

Send to

Choose Destination
Mycologia. 2014 Jul-Aug;106(4):676-85. doi: 10.3852/13-165. Epub 2014 Jun 3.

SNP markers identify widely distributed clonal lineages of Phytophthora colocasiae in Vietnam, Hawaii and Hainan Island, China.

Author information

1
Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, Tennessee.
2
Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, Tennessee, and China Agricultural University, Beijing.
3
National Center for Genome Resources, Santa Fe, New Mexico.
4
Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, Tennessee klamour@utk.edu.

Abstract

Taro (Colocasia esculenta) is an important food crop, and taro leaf blight caused by Phytophthora colocasiae can significantly affect production. Our objectives were to develop single nucleotide polymorphism (SNP) markers for P. colocasiae and characterize populations in Hawaii (HI), Vietnam (VN) and Hainan Island, China (HIC). In total, 379 isolates were analyzed for mating type and multilocus SNP profiles including 214 from HI, 97 from VN and 68 from HIC. A total of 1152 single nucleotide variant (SNV) sites were identified via restriction site-associated DNA (RAD) sequencing of two field isolates. Genotyping with 27 SNPs revealed 41 multilocus SNP genotypes grouped into seven clonal lineages containing 2-232 members. Three clonal lineages were shared among countries. In addition, five SNP markers had a low incidence of loss of heterozygosity (LOH) during asexual laboratory growth. For HI and VN, >95% of isolates were the A2 mating type. On HIC, isolates within single clonal lineages had A1, A2 and A0 (neuter) isolates. The implications for the wide dispersal of clonal lineages are discussed.

KEYWORDS:

SNP; loss of heterozygosity; population genetics

PMID:
24895424
DOI:
10.3852/13-165
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Taylor & Francis
Loading ...
Support Center