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J Genet Genomics. 2014 May 20;41(5):293-304. doi: 10.1016/j.jgg.2014.04.002. Epub 2014 Apr 26.

Comprehensive analysis of lipid composition in crude palm oil using multiple lipidomic approaches.

Author information

1
Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119275, Singapore.
2
Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119275, Singapore. Electronic address: bchmrw@nus.edu.sg.
3
State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: ghshui@genetics.ac.cn.

Abstract

Palm oil is currently the leading edible oil consumed worldwide. Triacylglycerol (TAG) and diacylglycerol (DAG) are the dominant lipid classes in palm oil. Other lipid classes present in crude palm oil, such as phospholipids and galactolipids, are very low in abundance. These low-abundance lipids constitute key intermediates in lipid biosynthesis. In this study, we applied multiple lipidomic approaches, including high-sensitivity and high-specificity multiple reaction monitoring, to comprehensively quantify individual lipid species in crude palm oil. We also established a new liquid chromatography-coupled mass spectrometry method that allows direct quantification of low-abundance galactolipids in palm oil without the need for sample pretreatment. As crude palm oil contains large amounts of neutral lipids, our direct-detection method circumvents many of the challenges encountered with conventional lipid quantification methods. This approach allows direct measurement of lipids with no hassle during sample preparation and is more accurate and precise compared with other methods.

KEYWORDS:

Galactolipids; Lipidomics; Mass spectrometry; Palm oil; Phospholipids

PMID:
24894356
DOI:
10.1016/j.jgg.2014.04.002
[Indexed for MEDLINE]

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