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J Food Sci. 2014 Jul;79(7):T1462-8. doi: 10.1111/1750-3841.12501. Epub 2014 Jun 3.

Metabolism of the hepatotoxic compound sophoraflavanone G in rat liver microsomes.

Author information

1
School of Pharmacy, Fudan Univ, Shanghai, China.

Abstract

Our study aimed at investigating the metabolic characteristics of sophoraflavanone G (SFG), one of the hepatotoxic constituents of Sophora flavescens, in rat liver microsomes (RLMs). SFG was metabolized to 3 phase I metabolites, di-hydroxylated SFG (M1), mono-hydroxylated SFG (M2), dehydrogenated product of mono-hydroxylated SFG (M3) and 3 SFG glucuronides (M4, M5, and M6) by RLMs. The formation kinetics of M2 conformed to biphasic kinetics in RLMs. The formation kinetics of M4 and M5 best-fitted the Hill equation kinetics. Chemical inhibition studies found that CYP1A2 and CYP2E1 were the major enzymes responsible for the formation of M2, and the formation of M4 and M5 may be catalyzed by multiple UGT1A isoforms.

KEYWORDS:

RLMs; hepatotoxicity; metabolism; sophoraflavanone G

PMID:
24894298
DOI:
10.1111/1750-3841.12501
[Indexed for MEDLINE]

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