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PLoS One. 2014 Jun 3;9(6):e98839. doi: 10.1371/journal.pone.0098839. eCollection 2014.

Impairments in the initiation of maternal behavior in oxytocin receptor knockout mice.

Author information

1
Laboratory of Neuroendocrinology and Behavior, Department of Biological Sciences, Kent State University, Kent, Ohio, United States of America; School of Biomedical Sciences, Kent State University, Kent, Ohio, United States of America.
2
Laboratory of Neuroendocrinology and Behavior, Department of Biological Sciences, Kent State University, Kent, Ohio, United States of America.
3
Department of Oral Microbiology, School of Dentistry and the Brain Science and Engineering Institute, Kyungpook National University, Daegu, South Korea.

Abstract

Oxytocin (Oxt) acting through its single receptor subtype, the Oxtr, is important for the coordination of physiology and behavior associated with parturition and maternal care. Knockout mouse models have been helpful in exploring the contributions of Oxt to maternal behavior, including total body Oxt knockout (Oxt -/-) mice, forebrain conditional Oxtr knockout (Oxtr FB/FB) mice, and total body Oxtr knockout (Oxtr -/-) mice. Since Oxtr -/- mice are unable to lactate, maternal behavior has only been examined in virgin females, or in dams within a few hours of parturition, and there have been no studies that have examined their anxiety-like and depression-like behavior following parturition. To improve our understanding of how the absence of Oxt signaling affects maternal behavior, mood and anxiety, we designed a study using Oxtr -/- mice that separated nursing behavior from other aspects of maternal care, such as licking and grooming by thelectomizing (i.e. removing the nipples) of Oxtr +/+ mice and sham-thelectomizing Oxtr -/- mice, and pairing both genotypes with a wet nurse. We then measured pup abandonment, maternal behavior, and postpartum anxiety-like and depression-like behaviors. We hypothesized that genetic disruption of the Oxtr would impact maternal care, mood and anxiety. Specifically, we predicted that Oxtr -/- dams would have impaired maternal care and increased anxiety-like and depression-like behaviors in the postpartum period. We found that Oxtr -/- dams had significantly higher levels of pup abandonment compared to controls, which is consistent with previous work in Oxtr FB/FB mice. Interestingly, Oxtr -/- dams that initiated maternal care did not differ from wildtype controls in measures of maternal behavior. We also did not find any evidence of altered anxiety-like or depressive-like behavior in the postpartum period of Oxtr -/- dams. Thus, our data suggest that Oxt lowers the threshold for the initiation of maternal behavior.

PMID:
24892749
PMCID:
PMC4044031
DOI:
10.1371/journal.pone.0098839
[Indexed for MEDLINE]
Free PMC Article

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