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Mediators Inflamm. 2014;2014:837107. doi: 10.1155/2014/837107. Epub 2014 May 7.

Glutamine supplementation attenuates expressions of adhesion molecules and chemokine receptors on T cells in a murine model of acute colitis.

Author information

1
Department of Surgery, National Taiwan University Hospital, Taipei 100, Taiwan.
2
School of Nutrition and Health Sciences, Taipei Medical University, Taipei 110, Taiwan.
3
Department of Surgery, National Taiwan University Hospital, Taipei 100, Taiwan ; Department of Primary Care Medicine, College of Medicine, National Taiwan University, Taipei 100, Taiwan.

Abstract

BACKGROUND:

Migration of T cells into the colon plays a major role in the pathogenesis in inflammatory bowel disease. This study investigated the effects of glutamine (Gln) supplementation on chemokine receptors and adhesion molecules expressed by T cells in mice with dextran sulfate sodium- (DSS-) induced colitis.

METHODS:

C57BL/6 mice were fed either a standard diet or a Gln diet replacing 25% of the total nitrogen. After being fed the diets for 5 days, half of the mice from both groups were given 1.5% DSS in drinking water to induce colitis. Mice were killed after 5 days of DSS exposure.

RESULTS:

DSS colitis resulted in higher expression levels of P-selectin glycoprotein ligand- (PSGL-) 1, leukocyte function-associated antigen- (LFA-) 1, and C-C chemokine receptor type 9 (CCR9) by T helper (Th) and cytotoxic T (Tc) cells, and mRNA levels of endothelial adhesion molecules in colons were upregulated. Gln supplementation decreased expressions of PSGL-1, LFA-1, and CCR9 by Th cells. Colonic gene expressions of endothelial adhesion molecules were also lower in Gln-colitis mice. Histological finding showed that colon infiltrating Th cells were less in the DSS group with Gln administration.

CONCLUSIONS:

Gln supplementation may ameliorate the inflammation of colitis possibly via suppression of T cell migration.

PMID:
24891768
PMCID:
PMC4033481
DOI:
10.1155/2014/837107
[Indexed for MEDLINE]
Free PMC Article

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