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Cold Spring Harb Protoc. 2014 Jun 2;2014(6):687-9. doi: 10.1101/pdb.prot080770.

3'-End labeling of RNA with yeast Poly(A) polymerase and 3'-deoxyadenosine 5'-[α-32P]triphosphate.

Abstract

This protocol is used to label RNA molecules (in vitro-synthesized or in vivo-purified RNA molecules) that have free 3'-hydroxyl termini. The reaction is performed in 10 min using yeast poly(A) polymerase and 3'-deoxyadenosine 5'-[α-(32)P]triphosphate (cordycepin 5'-[α-(32)P]triphosphate), a chain-terminating nucleotide. At the end of the procedure, the reaction is desalted by gel filtration to remove any unincorporated nucleotides.

PMID:
24890209
DOI:
10.1101/pdb.prot080770
[Indexed for MEDLINE]

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