Microarray-based resonance light scattering assay for detecting DNA methylation and human DNA methyltransferase simultaneously with high sensitivity

Analyst. 2014 Jul 21;139(14):3537-40. doi: 10.1039/c4an00336e.

Abstract

A microarray-based resonance light scattering assay, with the combination of methylation-sensitive endonuclease and gold nanoparticle (GNP) probes, has been proposed to sensitively distinguish the DNA methylation level as low as 0.01% (10 pM methylated DNA in 100 nM total DNA) and detect human DNA methyltransferase 1 (Dnmt1) down to 0.1 U mL(-1).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA (Cytosine-5-)-Methyltransferase 1
  • DNA (Cytosine-5-)-Methyltransferases / analysis*
  • DNA / analysis
  • DNA Methylation*
  • Enzyme Assays / instrumentation
  • Equipment Design
  • Gold / chemistry
  • Humans
  • Light
  • Limit of Detection
  • Metal Nanoparticles / chemistry
  • Microarray Analysis / instrumentation*
  • Scattering, Radiation

Substances

  • Gold
  • DNA
  • DNA (Cytosine-5-)-Methyltransferase 1
  • DNA (Cytosine-5-)-Methyltransferases
  • DNMT1 protein, human