Abstract
A microarray-based resonance light scattering assay, with the combination of methylation-sensitive endonuclease and gold nanoparticle (GNP) probes, has been proposed to sensitively distinguish the DNA methylation level as low as 0.01% (10 pM methylated DNA in 100 nM total DNA) and detect human DNA methyltransferase 1 (Dnmt1) down to 0.1 U mL(-1).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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DNA (Cytosine-5-)-Methyltransferase 1
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DNA (Cytosine-5-)-Methyltransferases / analysis*
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DNA / analysis
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DNA Methylation*
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Enzyme Assays / instrumentation
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Equipment Design
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Gold / chemistry
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Humans
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Light
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Limit of Detection
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Metal Nanoparticles / chemistry
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Microarray Analysis / instrumentation*
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Scattering, Radiation
Substances
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Gold
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DNA
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DNA (Cytosine-5-)-Methyltransferase 1
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DNA (Cytosine-5-)-Methyltransferases
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DNMT1 protein, human