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Microb Cell Fact. 2014 May 3;13:63. doi: 10.1186/1475-2859-13-63.

Current development in genetic engineering strategies of Bacillus species.

Author information

1
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China. zhang_dw@tib.cas.cn.

Abstract

The complete sequencing and annotation of the genomes of industrially-important Bacillus species has enhanced our understanding of their properties, and allowed advances in genetic manipulations in other Bacillus species. Post-genomic studies require simple and highly efficient tools to enable genetic manipulation. Here, we summarize the recent progress in genetic engineering strategies for Bacillus species. We review the available genetic tools that have been developed in Bacillus species, as well as methods developed in other species that may also be applicable in Bacillus. Furthermore, we address the limitations and challenges of the existing methods, and discuss the future research prospects in developing novel and useful tools for genetic modification of Bacillus species.

PMID:
24885003
PMCID:
PMC4030025
DOI:
10.1186/1475-2859-13-63
[Indexed for MEDLINE]
Free PMC Article

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