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Evid Based Complement Alternat Med. 2014;2014:916531. doi: 10.1155/2014/916531. Epub 2014 May 5.

An Immunomodulatory Protein (Ling Zhi-8) from a Ganoderma lucidum Induced Acceleration of Wound Healing in Rat Liver Tissues after Monopolar Electrosurgery.

Author information

1
School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan ; Research Center for Biomedical Implants and Microsurgery Devices, Taipei Medical University, Taipei 110, Taiwan.
2
Division of Oral Rehabilitation and Center of Pediatric Dentistry, Department of Dentistry, Taipei Medical University Hospital, Taipei 110, Taiwan ; Research Center for Biomedical Devices and Prototyping Production, Taipei Medical University, Taipei 110, Taiwan.
3
School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan ; Research Center for Biomedical Devices and Prototyping Production, Taipei Medical University, Taipei 110, Taiwan ; Department of Dentistry, Sijhih Cathay General Hospital, Taipei 221, Taiwan.
4
Research Center for Biomedical Devices and Prototyping Production, Taipei Medical University, Taipei 110, Taiwan ; School of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan.
5
School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan ; Division of Oral and Maxillofacial Surgery, Department of Dentistry, Taipei Medical University Hospital, Taipei, Taiwan.
6
Research Center for Biomedical Implants and Microsurgery Devices, Taipei Medical University, Taipei 110, Taiwan ; Research Center for Biomedical Devices and Prototyping Production, Taipei Medical University, Taipei 110, Taiwan ; Graduate Institute of Biomedical Materials and Tissue Engineering, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan ; Department of Dentistry, Taipei Medical University-Shuang Ho Hospital, Taipei 235, Taiwan.

Abstract

The purpose of this study was to investigate the effect of an immunomodulatory protein (Ling Zhi-8, LZ-8) on wound healing in rat liver tissues after monopolar electrosurgery. Animals were sacrificed for evaluations at 0, 3, 7, and 28 days postoperatively. It was found that the wound with the LZ-8 treatment significantly increases wound healing. Western blot analysis clearly indicated that the expression of NF-κB was decreased at 3, 7, and 28 days when liver tissues were treated with LZ-8. Moreover, caspase-3 activity of the liver tissue also significantly decreases at 7 and 28 days, respectively. DAPI staining and TUNEL assays revealed that only a minimal dispersion of NF-κB was found on the liver tissue treated with LZ-8 at day 7 as compared with day 3 and tissues without LZ-8 treatment. Similarly, apoptosis was decreased on liver tissues treated with LZ-8 at 7 days when compared to the control (monopolar electrosurgery) tissues. Therefore, the analytical results demonstrated that LZ-8 induced acceleration of wound healing in rat liver tissues after monopolar electrosurgery.

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