Objective: To observe the changes in subgingival microflora before and after Er:YAG laser treatment on diabetic patients with periodontitis, and to compare with the subgingival microflora of chronic periodontitis.
Methods: Subgingival plaque of 13 pairs of teeth (26 sites) was selected from type 2 diabetic patients at pretreatment, one month post-treatment, and three months post-treatment. Subgingival plaque was also obtained from 11 cases of moderate to severe chronic periodontitis with similar severity of periodontitis. The DNA of the subgingival plaque samples was extracted. Whole bacterial 16S rDNA gene fragments separated by denaturing gradient gel electrophoresis. Specific DNA bands were then chosen for retrieval and sequencing.
Results: The gene sequencing results of the special DNA bands of subgingival plaque samples show that the pathogenic bacteria of both diabetic periodontitis and simple chronic periodontitis were Prevotella intermedia and Tannerella forsythia, respectively. The composition of the subgingival microflora before and after laser treatment changed. Some DNA bands, including that of Tannerella forsythia, disappeared or weakened one month after treatment. A new strip appeared, which belonged to Actinomyces sp.
Conclusion: The profiles of the subgingival microflora changed after treatment, and one month was indicated as an important stage. Er:YAG laser may have an important function in delaying microflora recolonization.
目的: 动态观察2型糖尿病相关性牙周炎患者经Er: YAG激光治疗前后龈下菌群的变化，并与不伴全身疾病的慢性牙周炎龈下菌群进行比较。
方法: 收集11例2型糖尿病相关性牙周炎患者的13对患牙（26个位点）作为试验组，分别进行Er: YAG激光治疗和超声治疗，采集治疗前及治疗后1、3个月的龈下菌斑；同时收集11例牙周状况相近的不伴全身疾病的中重度慢性牙周炎患者13个位点的龈下菌斑作为对照组，分析试验组是否有菌群种类变化。提取龈下菌斑DNA，进行变性梯度凝胶电泳分离及条带回收测序
结果: 试验组与对照组患牙龈下菌斑的优势致病菌存在差异，分别为中间普雷沃菌和福赛斯坦纳菌。激光组治疗前后龈下菌群构成也发生改变，治疗后1个月，有的条带表达减弱或消失，并有新条带出现；测序结果表明，新出现的条带为放线菌的一种，减弱、消失的为福赛斯坦纳菌。
结论: Er: YAG激光治疗前后龈下菌群的构成发生变化，治疗后1个月是关键时期；治疗后3个月，激光治疗在阻止细菌再定植方面可能更具优势。