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Nat Biotechnol. 2014 Aug;32(8):819-21. doi: 10.1038/nbt.2925. Epub 2014 Jun 1.

Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system.

Author information

1
1] Biology of Host-Parasite Interactions Unit, Institut Pasteur, Paris, France. [2] CNRS URA 2581, Institut Pasteur, Paris, France. [3].
2
1] Biology of Host-Parasite Interactions Unit, Institut Pasteur, Paris, France. [2] CNRS URA 2581, Institut Pasteur, Paris, France.

Abstract

Genome manipulation in the malaria parasite Plasmodium falciparum remains largely intractable and improved genomic tools are needed to further understand pathogenesis and drug resistance. We demonstrated the CRISPR-Cas9 system for use in P. falciparum by disrupting chromosomal loci and generating marker-free, single-nucleotide substitutions with high efficiency. Additionally, an artemisinin-resistant strain was generated by introducing a previously implicated polymorphism, thus illustrating the value of efficient genome editing in malaria research.

PMID:
24880488
DOI:
10.1038/nbt.2925
[Indexed for MEDLINE]

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