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Arch Pathol Lab Med. 2014 Jun;138(6):828-32. doi: 10.5858/arpa.2013-0134-OA.

Study of circulating microRNA-125b levels in serum exosomes in advanced melanoma.

Author information

1
From the Laboratory of Biochemistry (Drs Alegre and González and Ms Rodriguez) and the Department of Medical Oncology (Drs Sanmamed and Martín-Algarra and Dr Carranza), University Clinic of Navarra, Pamplona, Navarra, Spain.

Abstract

CONTEXT:

Malignant melanoma is an aggressive tumor that produces exosomes, which contain microRNAs (miRNAs) that could be of utility in following tumoral cell dysregulation. MicroR-125b is a miRNA whose down-regulation seems to be implicated in melanoma progression.

OBJECTIVE:

To analyze miR-125b levels in serum, and in exosomes obtained from serum, from patients with advanced melanoma.

DESIGN:

Serum samples were obtained from 21 patients with advanced melanoma, from 16 disease-free patients with melanoma, and from 19 healthy volunteers. Exosomes were isolated from serum by precipitation, and miR-16 and miR-125b levels were quantified by real-time polymerase chain reaction.

RESULTS:

MicroR-16, but not miR-125b, was detected in all samples, and miR-16 levels were significantly higher in serum than they were in exosomes. MicroR-16 expression levels did not differ significantly between the 2 groups (patients with melanoma and healthy donors). There was a significant relationship between miR-125b and miR-16 levels in exosomes. Additionally, miR-125b levels in exosomes were significantly lower in patients with melanoma compared with disease-free patients with melanoma and healthy controls.

CONCLUSIONS:

Exosomes can provide a suitable material to measure circulating miRNA in melanoma, and miR-16 can be used as an endogenous normalizer. Lower levels of miR-125b in exosomes obtained from serum are associated with advanced melanoma disease, probably reflecting the tumoral cell dysregulation.

PMID:
24878024
DOI:
10.5858/arpa.2013-0134-OA
[Indexed for MEDLINE]

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