Format

Send to

Choose Destination
J Virol Methods. 2014 Sep;206:1-4. doi: 10.1016/j.jviromet.2014.05.010. Epub 2014 May 27.

Comparison of a conventional HIV 1/2 line immunoassay with a rapid confirmatory HIV 1/2 assay.

Author information

1
Blood Transfusion Service SRC Berne, Murtenstrasse 133, 3008 Berne, Switzerland.
2
Blood Transfusion Service SRC Berne, Murtenstrasse 133, 3008 Berne, Switzerland. Electronic address: christoph.niederhauser@bsd-be.ch.

Abstract

The performance of the rapid confirmatory HIV 1/2 assay Geenius was compared with the conventional HIV 1/2 line immunoblot (INNO-LIA HIV I/II Score). One hundred HIV 1/2 confirmed positive samples from donors and patients and 136 negative screening samples from blood donors were evaluated with both assays. A 20 member performance panel consisting of different HIV 1 and 2 subtypes was also analysed. Ninety-nine of the confirmed HIV positive samples were positive with both assays. One sample was positive with the INNO-LIA HIV I/II Score but indeterminate with the Geenius HIV 1/2. From 136 negative blood donor samples (negative with a combo HIV assay and a highly sensitive ID-NAT), 125 were concordant negative. Six and five samples were incorrectly indeterminate with the INNO-LIA HIV I/II Score and the Geenius HIV 1/2, respectively. One sample was weak positive with the INNO-LIA HIV I/II Score but negative with the Geenius HIV 1/2. The 20 member performance showed equivalent results with both assays. The rapid assay showed a comparable sensitivity and specificity for confirmation for positive and negative HIV donor and patient samples as well for a 20 member performance panel.

KEYWORDS:

Confirmation assays; HIV; Sensitivity; Specificity

PMID:
24877900
DOI:
10.1016/j.jviromet.2014.05.010
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center