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Biomed Res Int. 2014;2014:496219. doi: 10.1155/2014/496219. Epub 2014 Apr 27.

Novel molecular beacon probe-based real-time RT-PCR assay for diagnosis of Crimean-Congo hemorrhagic fever encountered in India.

Author information

1
High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, Bhopal 462022, India.

Abstract

Crimean-Congo hemorrhagic fever (CCHF) is an emerging zoonotic disease in India and requires immediate detection of infection both for preventing further transmission and for controlling the infection. The present study describes development, optimization, and evaluation of a novel molecular beacon-based real-time RT-PCR assay for rapid, sensitive, and specific diagnosis of Crimean-Congo hemorrhagic fever virus (CCHFV). The developed assay was found to be a better alternative to the reported TaqMan assay for routine diagnosis of CCHF.

PMID:
24877102
PMCID:
PMC4022296
DOI:
10.1155/2014/496219
[Indexed for MEDLINE]
Free PMC Article

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