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Biomed Res Int. 2014;2014:157923. doi: 10.1155/2014/157923. Epub 2014 Apr 29.

Evaluation of inhibitory effect of recreational drugs on dopaminergic terminal neuron by PET and whole-body autoradiography.

Author information

  • 1Department of Biomedical Imaging and Radiological Sciences, National Yang Ming University, Taipei 11221, Taiwan ; Department of Education and Research, Taipei City Hospital, Taipei 10341, Taiwan ; Biophotonic and Molecular Imaging Research Center, National Yang Ming University, Taipei 11221, Taiwan.
  • 2Department of Biomedical Imaging and Radiological Sciences, National Yang Ming University, Taipei 11221, Taiwan ; Division of Nuclear Medicine, Taipei City Hospital Zhongxiao Branch, Taipei 11556, Taiwan.
  • 3Department of Cancer Systems Imaging, The University of Texas MD Anderson Cancer Center, TX, USA.
  • 4Department of Biomedical Imaging and Radiological Sciences, National Yang Ming University, Taipei 11221, Taiwan ; Molecular and Genetic Imaging Core/Taiwan Mouse Clinic, National Comprehensive Mouse Phenotyping and Drug Testing Center, Taipei 11533, Taiwan ; National PET/Cyclotron Center and Department of Nuclear Medicine, Taipei Veterans General Hospital, Taipei 11221, Taiwan.
  • 5Department of Biomedical Imaging and Radiological Sciences, National Yang Ming University, Taipei 11221, Taiwan.
  • 6Nuclear Medicine Department and PET-CT Center, Shuang Ho Hospital Ministry of Health and Welfare, Taipei Medical University, New Taipei City 23561, Taiwan.
  • 7Department of Biomedical Imaging and Radiological Sciences, National Yang Ming University, Taipei 11221, Taiwan ; Biophotonic and Molecular Imaging Research Center, National Yang Ming University, Taipei 11221, Taiwan ; Molecular and Genetic Imaging Core/Taiwan Mouse Clinic, National Comprehensive Mouse Phenotyping and Drug Testing Center, Taipei 11533, Taiwan ; National PET/Cyclotron Center and Department of Nuclear Medicine, Taipei Veterans General Hospital, Taipei 11221, Taiwan ; Institute of Clinical Medicine, National Yang-Ming University, Taipei 11221, Taiwan.

Abstract

There is little investigation for the functional roles of peripheral dopamine. [(18)F]FDOPA has been used in cancer imaging (i.e., neuroendocrine and tumors pancreatic tumors) and neuroimaging (i.e., Parkinson's disease and Huntington's disease). Here, we accessed side effects of recreational drugs such as ketamine, cocaine, and methamphetamine on dopamine neurons in peripheral organs by using positron emission tomography (PET) imaging and quantitative whole-body autoradiography (QWBAR) with [(18)F]FDOPA. The images were applied for the measurement of specific binding ratios (SBRs) of striatum with the cerebellum as the reference region. Clear striatal [(18)F]FDOPA-derived radioactivity was observed. Moderate level of radiotracer accumulation was presented in the mucosal layers of the stomach and small intestine. The medulla layers of kidney had higher radioactivity than that of the cortex. Blocking images markedly eliminated the specific binding of [(18)F]FDOPA in the striatum and in peripheral organs such as stomachs, intestines, and kidney. Ketamine showed the highest inhibitory effect on striatal [(18)F]FDOPA-derived radioactivity followed by cocaine and methamphetamine. The current results demonstrated a useful crossing-validating tool that enhances the capability of [(18)F]FDOPA for further investigations of the alteration of dopaminergic neurons in the brain disorder or cancer diseases in peripheral tissues.

PMID:
24877059
PMCID:
PMC4022164
DOI:
10.1155/2014/157923
[PubMed - indexed for MEDLINE]
Free PMC Article
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