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Elife. 2014 May 29;3. doi: 10.7554/eLife.02811.

TAF7L modulates brown adipose tissue formation.

Author information

1
Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, United States.
2
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, China.
3
School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, Israel.

Abstract

Brown adipose tissue (BAT) plays an essential role in metabolic homeostasis by dissipating energy via thermogenesis through uncoupling protein 1 (UCP1). Previously, we reported that the TATA-binding protein associated factor 7L (TAF7L) is an important regulator of white adipose tissue (WAT) differentiation. In this study, we show that TAF7L also serves as a molecular switch between brown fat and muscle lineages in vivo and in vitro. In adipose tissue, TAF7L-containing TFIID complexes associate with PPARγ to mediate DNA looping between distal enhancers and core promoter elements. Our findings suggest that the presence of the tissue-specific TAF7L subunit in TFIID functions to promote long-range chromatin interactions during BAT lineage specification.

KEYWORDS:

BAT; DNA looping; PPARγ; UCP1; developmental biology; mouse; obesity; stem cells

PMID:
24876128
PMCID:
PMC4066819
DOI:
10.7554/eLife.02811
[Indexed for MEDLINE]
Free PMC Article

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