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Anaerobe. 2014 Aug;28:126-9. doi: 10.1016/j.anaerobe.2014.05.005. Epub 2014 May 27.

Development and evaluation of new primers for PCR-based identification of Prevotella intermedia.

Author information

1
Department of Periodontology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, 639 Zhi Zao Ju Road, Shanghai 210011, China.
2
Shanghai Research Institute of Stomatology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 210011, China.
3
Endodontics and Operative Dentistry, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 210011, China.
4
Department of Periodontology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, 639 Zhi Zao Ju Road, Shanghai 210011, China. Electronic address: shurong123@hotmail.com.

Abstract

The aim of this study was to develop new Prevotella intermedia-specific PCR primers based on the 16S rRNA. The new primer set, Pi-192 and Pi-468, increased the accuracy of PCR-based P. intermedia identification and could be useful in the detection of P. intermedia as well as epidemiological studies on periodontal disease.

KEYWORDS:

Prevotella intermedia; Species-specific PCR primers

PMID:
24875331
DOI:
10.1016/j.anaerobe.2014.05.005
[Indexed for MEDLINE]

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