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Methods. 2014 Aug 1;68(3):417-24. doi: 10.1016/j.ymeth.2014.05.007. Epub 2014 May 27.

FLP/FRT and Cre/lox recombination technology in C. elegans.

Author information

1
New York University School of Medicine, Skirball Institute of Biomolecular Medicine, Department of Pathology, and Helen L. and Martin S. Kimmel Center for Stem Cell Biology, 540 First Avenue, New York, NY 10016, USA. Electronic address: jane.hubbard@med.nyu.edu.

Abstract

One of the most powerful aspects of biological inquiry using model organisms is the ability to control gene expression. A holy grail is both temporal and spatial control of the expression of specific gene products - that is, the ability to express or withhold the activity of genes or their products in specific cells at specific times. Ideally such a method would also regulate the precise levels of gene activity, and alterations would be reversible. The related goal of controlled or purposefully randomized expression of visible markers is also tremendously powerful. While not all of these feats have been accomplished in Caenorhabditis elegans to date, much progress has been made, and recent technologies put these goals within closer reach. Here, I present published examples of successful two-component site-specific recombination in C. elegans. These technologies are based on the principle of controlled intra-molecular excision or inversion of DNA sequences between defined sites, as driven by FLP or Cre recombinases. I discuss several prospects for future applications of this technology.

KEYWORDS:

Excision; Heat-shock; Inversion; Recombinase; Two-component system

PMID:
24874786
PMCID:
PMC4210360
DOI:
10.1016/j.ymeth.2014.05.007
[Indexed for MEDLINE]
Free PMC Article

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