Format

Send to

Choose Destination
Acta Biomater. 2014 Aug;10(8):3733-46. doi: 10.1016/j.actbio.2014.05.020. Epub 2014 May 27.

Cotton-wool-like bioactive glasses for bone regeneration.

Author information

1
Department of Materials, Imperial College London, South Kensington, London SW7 2AZ, UK. Electronic address: gowsh.p@imperial.ac.uk.
2
Department of Materials, Imperial College London, South Kensington, London SW7 2AZ, UK.
3
Department of Frontier Materials, Nagoya Institute of Technology, Nagoya 466-8555, Japan.
4
School of Materials, The University of Manchester, Oxford Rd., Manchester M13 9PL, UK.
5
Department of Materials, Imperial College London, South Kensington, London SW7 2AZ, UK; Department of Bioengineering, Imperial College London, South Kensington, London SW7 2AZ, UK.

Abstract

Inorganic sol-gel solutions were electrospun to produce the first bioactive three-dimensional (3-D) scaffolds for bone tissue regeneration with a structure like cotton-wool (or cotton candy). This flexible 3-D fibrous structure is ideal for packing into complex defects. It also has large inter-fiber spaces to promote vascularization, penetration of cells and transport of nutrients throughout the scaffold. The 3-D fibrous structure was obtained by electrospinning, where the applied electric field and the instabilities exert tremendous force on the spinning jet, which is required to be viscoelastic to prevent jet break up. Previously, polymer binding agents were used with inorganic solutions to produce electrospun composite two-dimensional fibermats, requiring calcination to remove the polymer. This study presents novel reaction and processing conditions for producing a viscoelastic inorganic sol-gel solution that results in fibers by the entanglement of the intermolecularly overlapped nanosilica species in the solution, eliminating the need for a binder. Three-dimensional cotton-wool-like structures were only produced when solutions containing calcium nitrate were used, suggesting that the charge of the Ca(2+) ions had a significant effect. The resulting bioactive silica fibers had a narrow diameter range of 0.5-2μm and were nanoporous. A hydroxycarbonate apatite layer was formed on the fibers within the first 12h of soaking in simulated body fluid. MC3T3-E1 preosteoblast cells cultured on the fibers showed no adverse cytotoxic effect and they were observed to attach to and spread in the material.

KEYWORDS:

3-D cotton-wool-like structure; Bone regeneration scaffold; Electrospinning; Inorganic fibers; Sol–gel

PMID:
24874652
DOI:
10.1016/j.actbio.2014.05.020
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center