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Sci Rep. 2014 May 29;4:5114. doi: 10.1038/srep05114.

Protein patterns of black fungi under simulated Mars-like conditions.

Author information

1
University of Natural Resources and Life Sciences, Department of Biotechnology, Muthgasse 18, A - 1190 Vienna, Austria.
2
German Aerospace Center, Institute of Planetary Research, Rutherfordstrasse 2, D-12489 Berlin, Germany.

Abstract

Two species of microcolonial fungi - Cryomyces antarcticus and Knufia perforans - and a species of black yeasts-Exophiala jeanselmei - were exposed to thermo-physical Mars-like conditions in the simulation chamber of the German Aerospace Center. In this study the alterations at the protein expression level from various fungi species under Mars-like conditions were analyzed for the first time using 2D gel electrophoresis. Despite of the expectations, the fungi did not express any additional proteins under Mars simulation that could be interpreted as stress induced HSPs. However, up-regulation of some proteins and significant decreasing of protein number were detected within the first 24 hours of the treatment. After 4 and 7 days of the experiment protein spot number was increased again and the protein patterns resemble the protein patterns of biomass from normal conditions. It indicates the recovery of the metabolic activity under Martian environmental conditions after one week of exposure.

PMID:
24870977
PMCID:
PMC4037706
DOI:
10.1038/srep05114
[Indexed for MEDLINE]
Free PMC Article

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