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J Exp Bot. 2014 Aug;65(15):4065-95. doi: 10.1093/jxb/eru191. Epub 2014 May 27.

Linking chlorophyll a fluorescence to photosynthesis for remote sensing applications: mechanisms and challenges.

Author information

1
Department of Forest Sciences, University of Helsinki, PO Box 27, 00014 Helsinki, Finland joan.porcar@helsinki.fi.
2
Molecular Plant Biology, Department of Biochemistry, University of Turku, FI-20014 Turku, Finland.
3
Department of Forest Sciences, University of Helsinki, PO Box 27, 00014 Helsinki, Finland.
4
Faculty of ITC, University of Twente, PO Box 217, 7524 AE Enschede, The Netherlands.
5
Plant Biology under Mediterranean Conditions, Universitat de les Illes Balears, Ctra. de Valldemossa Km. 7.5, 07122 Palma, Spain.
6
Heinz Walz GmbH, Eichenring 6, D-91090 Effeltrich, Germany.
7
Department of Earth Physics and Thermodynamics, Faculty of Physics, University of Valencia, C/ Dr. Moliner, 50, 46100 Burjassot, Valencia, Spain.
8
Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109, USA.
9
Department of Global Ecology, Carnegie Institution of Washington, Stanford, CA 94305, USA.

Erratum in

Abstract

Chlorophyll a fluorescence (ChlF) has been used for decades to study the organization, functioning, and physiology of photosynthesis at the leaf and subcellular levels. ChlF is now measurable from remote sensing platforms. This provides a new optical means to track photosynthesis and gross primary productivity of terrestrial ecosystems. Importantly, the spatiotemporal and methodological context of the new applications is dramatically different compared with most of the available ChlF literature, which raises a number of important considerations. Although we have a good mechanistic understanding of the processes that control the ChlF signal over the short term, the seasonal link between ChlF and photosynthesis remains obscure. Additionally, while the current understanding of in vivo ChlF is based on pulse amplitude-modulated (PAM) measurements, remote sensing applications are based on the measurement of the passive solar-induced chlorophyll fluorescence (SIF), which entails important differences and new challenges that remain to be solved. In this review we introduce and revisit the physical, physiological, and methodological factors that control the leaf-level ChlF signal in the context of the new remote sensing applications. Specifically, we present the basis of photosynthetic acclimation and its optical signals, we introduce the physical and physiological basis of ChlF from the molecular to the leaf level and beyond, and we introduce and compare PAM and SIF methodology. Finally, we evaluate and identify the challenges that still remain to be answered in order to consolidate our mechanistic understanding of the remotely sensed SIF signal.

KEYWORDS:

GPP; Gross primary production; PAM; PSI; PSII; PSII connectivity; SIF.; leaf level; photosynthesis dynamics; photosystem I; photosystem II; pulse amplitude modulation; remote sensing; solar-induced fluorescence; sun-induced fluorescence

PMID:
24868038
DOI:
10.1093/jxb/eru191
[Indexed for MEDLINE]

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