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Arq Bras Endocrinol Metabol. 2014 Apr;58(3):232-6.

[Analysis of an iodide radioimmunoassay for 11-deoxicortisol measurement].

[Article in Portuguese]

Author information

  • 1Laboratório de Hormônios e Genética Molecular (LIM/42), Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil.

Abstract

OBJECTIVE:

Our aim was to correlate 11-deoxycortisol levels obtained by two currently available techniques for 11-deoxycortisol measurement: radioimmunoassay, and high performance liquid chromatography followed by tandem mass spectrometry (MS/MS). The latter is the gold standard method for steroid hormone measurement.

MATERIALS AND METHODS:

We selected 88 samples and the results of these two methods were compared by Deming regression.

RESULTS:

The analytical sensitivity of the RIA was 0.30 ng/mL, with inadequate linearity and inadequate precision profile (34% of the samples had a CV ≥ 20%). From the selected samples, 54 had measurable levels of 11-deoxycortisol in both methods and were used in the comparison. The comparison of RIA with LC-MS/MS showed an overestimation of the results by RIA. The correlation coefficient was 0.610; linear regression slope was 3.751; and the intercept was 0.145, indicating a poor correlation between the two methods.

CONCLUSION:

We concluded that 11-deoxycortisol measured by radioimmunoassay, despite a good analytical sensitivity, showed very low specificity, precluding its use as a reliable method for 11-deoxycortisol measurement.

PMID:
24863084
[PubMed - indexed for MEDLINE]
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