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Rev Soc Bras Med Trop. 2014 Mar-Apr;47(2):231-4.

Molecular identification of the agent of Q fever - Coxiella burnetii - in domestic animals in State of Rio de Janeiro, Brazil.

Author information

1
Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
2
Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
3
Secretaria de Saúde do Município de Itaboraí, Itaborarí, RJ.

Abstract

INTRODUCTION:

Over the last recent years, the number of Q fever cases have has increased throughout the world. An epidemiological investigation was performed in the area in which the first molecular documentation of Q fever in Brazil was previously reported.

METHODS:

Indirect immunofluorescence assay (IFA) and PCR of Coxiella burnetii targeting the htpAB gene were performed in samples from 14 dogs (blood); 1 cat (blood); 10 goats (blood, milk, vaginal swab and anal swab); 3 sheep (blood); and 2 horses (blood).

RESULTS:

Two dogs, two sheep and five goats were seroreactive. DNA was amplified from 6 milk and 2 blood samples from goats and from dogs, respectively. The sequence of the amplicons exhibited 99% sequence similarity with the homologous sequence of the htpAB gene of C. burnetii RSA 331 (GenBank - CP000890).

CONCLUSIONS:

The results confirm C. burnetii infection in animals in Rio de Janeiro and reinforce the need for the surveillance of Q fever in Brazil.

PMID:
24861300
[Indexed for MEDLINE]
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