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J Biol Chem. 2014 Jul 4;289(27):19098-109. doi: 10.1074/jbc.M114.571414. Epub 2014 May 23.

Bacterial RTX toxins allow acute ATP release from human erythrocytes directly through the toxin pore.

Author information

1
From the Department of Biomedicine, MEMBRANES, and.
2
Department of Dentistry.
3
From the Department of Biomedicine.
4
the Department of Molecular Biology and Genetics, Interdisciplinary Nanoscience Center (iNANO), Center for Insoluble Proteins (inSPIN), Aarhus University, Aarhus C 8000, Denmark.
5
From the Department of Biomedicine, MEMBRANES, and hp@fi.au.dk.

Abstract

ATP is as an extracellular signaling molecule able to amplify the cell lysis inflicted by certain bacterial toxins including the two RTX toxins α-hemolysin (HlyA) from Escherichia coli and leukotoxin A (LtxA) from Aggregatibacter actinomycetemcomitans. Inhibition of P2X receptors completely blocks the RTX toxin-induced hemolysis over a larger concentration range. It is, however, at present not known how the ATP that provides the amplification is released from the attacked cells. Here we show that both HlyA and LtxA trigger acute release of ATP from human erythrocytes that preceded and were not caused by cell lysis. This early ATP release did not occur via previously described ATP-release pathways in the erythrocyte. Both HlyA and LtxA were capable of triggering ATP release in the presence of the pannexin 1 blockers carbenoxolone and probenecid, and the HlyA-induced ATP release was found to be similar in erythrocytes from pannexin 1 wild type and knock-out mice. Moreover, the voltage-dependent anion channel antagonist TRO19622 had no effect on ATP release by either of the toxins. Finally, we showed that both HlyA and LtxA were able to release ATP from ATP-loaded lipid (1-palmitoyl-2-oleoyl-phosphatidylcholine) vesicles devoid of any erythrocyte channels or transporters. Again we were able to show that this happened in a non-lytic fashion, using calcein-containing vesicles as controls. These data show that both toxins incorporate into lipid vesicles and allow ATP to be released. We suggest that both toxins cause acute ATP release by letting ATP pass the toxin pores in both human erythrocytes and artificial membranes.

KEYWORDS:

ATP Release; Bacterial Toxin; Erythrocyte; Escherichia coli (E. coli); Hemolysin; Hemolysis; Leukotoxin; P2-receptors; Pannexin; Phospholipid Vesicle

PMID:
24860098
PMCID:
PMC4081947
DOI:
10.1074/jbc.M114.571414
[Indexed for MEDLINE]
Free PMC Article

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