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Nat Methods. 2014 Jul;11(7):731-3. doi: 10.1038/nmeth.2972. Epub 2014 May 25.

Fluorogenic probes for live-cell imaging of the cytoskeleton.

Author information

1
1] Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. [2].
2
1] Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. [2] National Centre of Competence of Research in Chemical Biology, Lausanne, Switzerland. [3].
3
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
4
Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
5
Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich Schiller University, Jena, Germany.
6
Bioimaging and Optics Platform, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
7
Max Planck Institute of Molecular Physiology, Dortmund, Germany.
8
Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna, Austria.
9
1] Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. [2] National Centre of Competence of Research in Chemical Biology, Lausanne, Switzerland.

Abstract

We introduce far-red, fluorogenic probes that combine minimal cytotoxicity with excellent brightness and photostability for fluorescence imaging of actin and tubulin in living cells. Applied in stimulated emission depletion (STED) microscopy, they reveal the ninefold symmetry of the centrosome and the spatial organization of actin in the axon of cultured rat neurons with a resolution unprecedented for imaging cytoskeletal structures in living cells.

PMID:
24859753
DOI:
10.1038/nmeth.2972
[Indexed for MEDLINE]

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