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Oncol Rep. 2014 Jul;32(1):173-80. doi: 10.3892/or.2014.3206. Epub 2014 May 22.

Curcumin suppresses cell proliferation through inhibition of the Wnt/β-catenin signaling pathway in medulloblastoma.

Author information

1
Department of Pathophysiology, Chongqing Medical University, Chongqing, P.R. China.
2
Department of Pathology, Insititute of Neuroscience, Chongqing Medical University, Chongqing, P.R. China.
3
Experimental and Teaching Center, Chongqing Medical University, Chongqing, P.R. China.
4
Institute of Pathology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University, Chongqing, P.R. China.
5
Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong, Hong Kong, SAR, P.R. China.

Abstract

Recently, the survival rate of medulloblastoma patients has greatly improved; yet, patients undergoing current treatment regimes suffer from serious therapy-related side-effects. The aim of the present study was to investigate the anticancer effects of curcumin on medulloblastoma cells by testing its capacity to suppress proliferation and regulate the Wnt/β-catenin pathway. In the present study, cell proliferation was determined by MTT assay. Cell cycle was observed by flow cytometry. The changes in the Wnt/β-catenin pathway were analyzed by immunofluorescence, western blot analysis and RT-PCR. Curcumin treatment resulted in a dose- and time-dependent inhibition of proliferation in the medulloblastoma cell line. Curcumin treatment arrested the cell-cycle at the G2/M phase. Furthermore, curcumin treatment led to activation of GSK-3β, reduced expression of β-catenin and its downstream target cyclin D1. The attenuation of the Wnt/β‑catenin pathway was due to the loss of nuclear β-catenin. In conclusion, curcumin can inhibit cell growth by suppressing the Wnt/β-catenin signaling pathway, and it has the potential to be developed as a therapeutic agent for medulloblastoma.

PMID:
24858998
DOI:
10.3892/or.2014.3206
[Indexed for MEDLINE]

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