Format

Send to

Choose Destination
J Ethnopharmacol. 2014 Aug 8;155(1):154-64. doi: 10.1016/j.jep.2014.04.053. Epub 2014 May 20.

The medicinal fungus Antrodia cinnamomea suppresses inflammation by inhibiting the NLRP3 inflammasome.

Author information

1
Center for Molecular and Clinical Immunology, Chang Gung University, Taoyuan 333, Taiwan; Department of Medical Biotechnology and Laboratory Sciences, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan; Laboratory of Nanomaterials, Chang Gung University, Taoyuan 333, Taiwan; Research Center of Bacterial Pathogenesis, Chang Gung University, Taoyuan 333, Taiwan.
2
Department of Medical Biotechnology and Laboratory Sciences, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan.
3
Center for Molecular and Clinical Immunology, Chang Gung University, Taoyuan 333, Taiwan; Department of Molecular Cell Biology and Health Sciences Research Institute, University of California, Merced, Merced, CA 95343, USA.
4
Biochemical Engineering Research Center, Ming Chi University of Technology, Taipei 24301, Taiwan; Chang Gung Biotechnology Corporation, Taipei 10508, Taiwan.
5
Cancer Research Center, National Cheng Kung University Hospital, Tainan 704, Taiwan.
6
Center for Molecular and Clinical Immunology, Chang Gung University, Taoyuan 333, Taiwan; Laboratory of Nanomaterials, Chang Gung University, Taoyuan 333, Taiwan; Research Center of Bacterial Pathogenesis, Chang Gung University, Taoyuan 333, Taiwan.
7
Department of Respiratory Therapy, Fu Jen Catholic University, Taipei 24205, Taiwan.
8
Center for Molecular and Clinical Immunology, Chang Gung University, Taoyuan 333, Taiwan; Laboratory of Nanomaterials, Chang Gung University, Taoyuan 333, Taiwan.
9
Center for Molecular and Clinical Immunology, Chang Gung University, Taoyuan 333, Taiwan; Laboratory of Nanomaterials, Chang Gung University, Taoyuan 333, Taiwan; Biochemical Engineering Research Center, Ming Chi University of Technology, Taipei 24301, Taiwan; Chang Gung Biotechnology Corporation, Taipei 10508, Taiwan; Laboratory of Cellular Physiology and Immunology, Rockefeller University, New York, NY 10021, USA. Electronic address: dingeyoung@hotmail.com.
10
Center for Molecular and Clinical Immunology, Chang Gung University, Taoyuan 333, Taiwan; Department of Medical Biotechnology and Laboratory Sciences, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan; Research Center of Bacterial Pathogenesis, Chang Gung University, Taoyuan 333, Taiwan. Electronic address: hclai@mail.cgu.edu.tw.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Antrodia cinnamomea--a medicinal fungus that is indigenous to Taiwan--has been used as a health tonic by aboriginal tribes and the Asian population. Recent studies indicate that Antrodia cinnamomea extracts exhibit hepato-protective, anti-hypertensive, anti-oxidative, anti-inflammatory, immuno-modulatory, and anti-cancer effects on cultured cells and laboratory animals. This study aims to explore the anti-inflammatory activity of an Antrodia cinnamomea ethanol extract (ACEE) and elucidate its underlying mechanisms of action using lipopolysaccharide (LPS)-primed, ATP-stimulated human THP-1 macrophages.

MATERIALS AND METHODS:

The effects of ACEE on cell viability were studied using the MTT assay. The expressions of genes, proteins, and pro-inflammatory cytokines were measured by quantitative real-time RT-PCR, Western blotting and ELISA, respectively. The ACEE was further investigated for its effects on reactive oxygen species (ROS) production using ROS detection kit.

RESULTS:

Our results showed that ACEE significantly inhibits ATP-induced secretion of interleukin-1β (IL-1β), interleukin-18 (IL-18) and tumor necrosis factor-α (TNF-α) by LPS-primed macrophages. ACEE also suppresses the transcription and activation of caspase-1, which is responsible for the cleavage and activation of IL-1β and IL-18. Of note, ACEE not only reduces expression of the inflammasome component NLRP3 and the purinergic receptor P2X7R but also inhibits ATP-induced ROS production and caspase-1 activation. Furthermore, the anti-inflammatory properties of ACEE correlate with reduced activation of the MAPK and NF-κB pathways.

CONCLUSION:

The results of the present study indicate that Antrodia cinnamomea suppresses the secretion of IL-1β and IL-18 associated with inhibition of the NLRP3 inflammasome in macrophages. These findings suggest that ACEE may have therapeutic potential for the treatment of inflammatory diseases.

KEYWORDS:

Anti-inflammatory activity; Antrodia cinnamomea; Caspase-1; Inflammasome; NLRP3; Reactive oxygen species

PMID:
24858059
DOI:
10.1016/j.jep.2014.04.053
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center