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Mar Drugs. 2014 May 22;12(5):3046-59. doi: 10.3390/md12053046.

Production of induced secondary metabolites by a co-culture of sponge-associated actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163.

Author information

1
Eskitis Institute for Drug Discovery, Griffith University, Brisbane, QLD 4111, Australia. yousef.dashti@griffithuni.edu.au.
2
Eskitis Institute for Drug Discovery, Griffith University, Brisbane, QLD 4111, Australia. t.grkovic@griffith.edu.au.
3
Department of Botany II, Julius-von-Sachs Institute for Biological Sciences, University of Würzburg, Julius-von-Sachs-Platz 3, D-97082 Würzburg, Germany. usama.ramadan@uni-wuerzburg.de.
4
Department of Botany II, Julius-von-Sachs Institute for Biological Sciences, University of Würzburg, Julius-von-Sachs-Platz 3, D-97082 Würzburg, Germany. ute.hentschel@uni-wuerzburg.de.
5
Eskitis Institute for Drug Discovery, Griffith University, Brisbane, QLD 4111, Australia. r.quinn@griffith.edu.au.

Abstract

Two sponge-derived actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163, were grown in co-culture and the presence of induced metabolites monitored by ¹H NMR. Ten known compounds, including angucycline, diketopiperazine and β-carboline derivatives 1-10, were isolated from the EtOAc extracts of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163. Co-cultivation of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163 induced the biosynthesis of three natural products that were not detected in the single culture of either microorganism, namely N-(2-hydroxyphenyl)-acetamide (11), 1,6-dihydroxyphenazine (12) and 5a,6,11a,12-tetrahydro-5a,11a-dimethyl[1,4]benzoxazino[3,2-b][1,4]benzoxazine (13a). When tested for biological activity against a range of bacteria and parasites, only the phenazine 12 was active against Bacillus sp. P25, Trypanosoma brucei and interestingly, against Actinokineospora sp. EG49. These findings highlight the co-cultivation approach as an effective strategy to access the bioactive secondary metabolites hidden in the genomes of marine actinomycetes.

PMID:
24857962
PMCID:
PMC4052330
DOI:
10.3390/md12053046
[Indexed for MEDLINE]
Free PMC Article

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