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Int J Biol Macromol. 2014 Aug;69:12-9. doi: 10.1016/j.ijbiomac.2014.05.027. Epub 2014 May 20.

Screening and comparison of antioxidant activities of polysaccharides from Coriolus versicolor.

Author information

  • 1Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China.
  • 2Heilongjiang Institute for Drug Control, Harbin 150001, China.
  • 3Chemistry Department, Henan Key Laboratory of Chemical Biology and Organic Chemistry, Key Laboratory of Applied Chemistry of Henan Universities, Zhengzhou University, Zhengzhou 450052, China.
  • 4Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China. Electronic address: qhwang668@sina.com.
  • 5Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China. Electronic address: hxkuang@hotmail.com.

Abstract

Six polysaccharide fractions (Coriolus versicolor polysaccharides: CVPS-1, CVPS-2, CVPS-3, CVPS-4, CVPS-5 and CVPS-6) were isolated and purified from the fruiting bodies of C. versicolor by ion exchange chromatography and gel chromatography. Their chemical and physical characteristics were determined by chemical methods, high performance liquid chromatography, and high-performance gel-permeation chromatography. Finally, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assay, superoxide radical assay, and hydroxyl radical assay were carried out to test the antioxidant activities of CVPS in vitro. The results indicated that the six CVPS fractions were acidic heteropolysaccharides, composed of mannose, rhamnose, glucuronic acid, glucose and fructose with different ratios. The molecular weights of CVPS-1, CVPS-2, CVPS-3, CVPS-4, CVPS-5 and CVPS-6 were 1740, 1480, 568, 880, 1260 and 1840kDa and the protein contents were 4.2%, 6.4%, 8.5%, 7.8%, 6.5% and 3.9%, respectively. Among the six fractions, CVPS with lower molecular weight, higher protein content and larger uronic acid amount, basically exhibited higher radical scavenging effects at the same concentration. Compared with other fractions, CVPS-3 exhibited the highest antioxidant activities. The effects of the molecular weight, protein content and uronic acid amount of the polysaccharides appeared to be significant on the improvement of the bioactivities.

KEYWORDS:

Antioxidant activities; Comparison; Coriolus versicolor polysaccharides

PMID:
24857871
DOI:
10.1016/j.ijbiomac.2014.05.027
[PubMed - indexed for MEDLINE]
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