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Trends Biotechnol. 2014 Jul;32(7):381-8. doi: 10.1016/j.tibtech.2014.04.008. Epub 2014 May 21.

Force-controlled manipulation of single cells: from AFM to FluidFM.

Author information

1
Institute of Microbiology, ETH Zurich, Vladimir-Prelog-Weg 4, 8093 Zurich, Switzerland.
2
Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, Gloriastrasse 35, 8092 Zurich, Switzerland.
3
DFG-Center for Functional Nanostructures, Karlsruhe Institute for Technology, Wolfgang-Gaede-Strasse 1a, 76131 Karlsruhe, Germany.
4
Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, Gloriastrasse 35, 8092 Zurich, Switzerland. Electronic address: zambelli@biomed.ee.ethz.ch.
5
Institute of Microbiology, ETH Zurich, Vladimir-Prelog-Weg 4, 8093 Zurich, Switzerland. Electronic address: vorholt@micro.biol.ethz.ch.

Abstract

The ability to perturb individual cells and to obtain information at the single-cell level is of central importance for addressing numerous biological questions. Atomic force microscopy (AFM) offers great potential for this prospering field. Traditionally used as an imaging tool, more recent developments have extended the variety of cell-manipulation protocols. Fluidic force microscopy (FluidFM) combines AFM with microfluidics via microchanneled cantilevers with nano-sized apertures. The crucial element of the technology is the connection of the hollow cantilevers to a pressure controller, allowing their operation in liquid as force-controlled nanopipettes under optical control. Proof-of-concept studies demonstrated a broad spectrum of single-cell applications including isolation, deposition, adhesion and injection in a range of biological systems.

KEYWORDS:

atomic force microscopy; fluidic force microscopy; single-cell analysis; single-cell perturbation

PMID:
24856959
DOI:
10.1016/j.tibtech.2014.04.008
[Indexed for MEDLINE]

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