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Biophys J. 2014 May 20;106(10):2243-53. doi: 10.1016/j.bpj.2014.04.001.

Fluid shear stress increases neutrophil activation via platelet-activating factor.

Author information

1
Department of Biomedical Engineering, Cornell University, Ithaca, New York.
2
Department of Biomedical Engineering, Cornell University, Ithaca, New York. Electronic address: mike.king@cornell.edu.

Abstract

Leukocyte exposure to hemodynamic shear forces is critical for physiological functions including initial adhesion to the endothelium, the formation of pseudopods, and migration into tissues. G-protein coupled receptors on neutrophils, which bind to chemoattractants and play a role in neutrophil chemotaxis, have been implicated as fluid shear stress sensors that control neutrophil activation. Recently, exposure to physiological fluid shear stresses observed in the microvasculature was shown to reduce neutrophil activation in the presence of the chemoattractant formyl-methionyl-leucyl-phenylalanine. Here, however, human neutrophil preexposure to uniform shear stress (0.1-2.75 dyn/cm(2)) in a cone-and-plate viscometer for 1-120 min was shown to increase, rather than decrease, neutrophil activation in the presence of platelet activating factor (PAF). Fluid shear stress exposure increased PAF-induced neutrophil activation in terms of L-selectin shedding, αMβ2 integrin activation, and morphological changes. Neutrophil activation via PAF was found to correlate with fluid shear stress exposure, as neutrophil activation increased in a shear stress magnitude- and time-dependent manner. These results indicate that fluid shear stress exposure increases neutrophil activation by PAF, and, taken together with previous observations, differentially controls how neutrophils respond to chemoattractants.

PMID:
24853753
PMCID:
PMC4052238
DOI:
10.1016/j.bpj.2014.04.001
[Indexed for MEDLINE]
Free PMC Article

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