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Br J Cancer. 2014 Jun 10;110(12):2985-95. doi: 10.1038/bjc.2014.238. Epub 2014 May 22.

Multi-colour FISH in oesophageal adenocarcinoma-predictors of prognosis independent of stage and grade.

Author information

1
1] Department of Pathology, Friedrich-Alexander Universität Erlangen-Nürnberg, Krankenhausstrasse 8/10, 91054 Erlangen, Germany [2] Comprehensive Cancer Center Erlangen-European Metropolitan Region Nuremberg 91054 Erlangen, Germany.
2
Department of Pathology, University Hospital Regensburg, 93053 Regensburg, Germany.
3
Department of Pathology and Cytology, 80992 Munich, Germany.
4
Department of Pathology, University Bern, CH 3010 Bern, Switzerland.
5
Department of Surgery, Klinikum Rechts der Isar, Technical University Munich, 81675 Munich, Germany.
6
Ventana Medical Systems, Inc., Oro Valley, AZ 85755, USA.
7
Abbott Molecular, Des Plaines, IL 60018, USA.

Abstract

BACKGROUND:

Oesophageal adenocarcinoma or Barrett's adenocarcinoma (EAC) is increasing in incidence and stratification of prognosis might improve disease management. Multi-colour fluorescence in situ hybridisation (FISH) investigating ERBB2, MYC, CDKN2A and ZNF217 has recently shown promising results for the diagnosis of dysplasia and cancer using cytological samples.

METHODS:

To identify markers of prognosis we targeted four selected gene loci using multi-colour FISH applied to a tissue microarray containing 130 EAC samples. Prognostic predictors (P1, P2, P3) based on genomic copy numbers of the four loci were statistically assessed to stratify patients according to overall survival in combination with clinical data.

RESULTS:

The best stratification into favourable and unfavourable prognoses was shown by P1, percentage of cells with less than two ZNF217 signals; P2, percentage of cells with fewer ERBB2- than ZNF217 signals; and P3, overall ratio of ERBB2-/ZNF217 signals. Median survival times for P1 were 32 vs 73 months, 28 vs 73 months for P2; and 27 vs 65 months for P3. Regarding each tumour grade P2 subdivided patients into distinct prognostic groups independently within each grade, with different median survival times of at least 35 months.

CONCLUSIONS:

Cell signal number of the ERBB2 and ZNF217 loci showed independence from tumour stage and differentiation grade. The prognostic value of multi-colour FISH-assays is applicable to EAC and is superior to single markers.

PMID:
24853183
PMCID:
PMC4056055
DOI:
10.1038/bjc.2014.238
[Indexed for MEDLINE]
Free PMC Article
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