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Int J Mol Sci. 2014 May 19;15(5):8821-34. doi: 10.3390/ijms15058821.

Antibody-conjugated paramagnetic nanobeads: kinetics of bead-cell binding.

Author information

1
Department of Pathobiology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand. swaseem92@yahoo.com.
2
Department of Physics, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand. maa5652@gmail.com.
3
Institute for Innovative Learning, Mahidol University, 999 Phuttamonthon 4 Road, Salaya, Nakorn Pathom 73170, Thailand. stefan-schreier@web.de.
4
Department of Pathobiology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand. rachanee.udo@mahidol.ac.th.
5
Department of Pathobiology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand. sbhakdi@gmail.com.

Abstract

Specific labelling of target cell surfaces using antibody-conjugated paramagnetic nanobeads is essential for efficient magnetic cell separation. However, studies examining parameters determining the kinetics of bead-cell binding are scarce. The present study determines the binding rates for specific and unspecific binding of 150 nm paramagnetic nanobeads to highly purified target and non-target cells. Beads bound to cells were enumerated spectrophotometrically. Results show that the initial bead-cell binding rate and saturation levels depend on initial bead concentration and fit curves of the form A(1 - exp(-kt)). Unspecific binding within conventional experimental time-spans (up to 60 min) was not detectable photometrically. For CD3-positive cells, the probability of specific binding was found to be around 80 times larger than that of unspecific binding.

PMID:
24852940
PMCID:
PMC4057761
DOI:
10.3390/ijms15058821
[Indexed for MEDLINE]
Free PMC Article

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