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ACS Appl Mater Interfaces. 2014 Dec 24;6(24):21788-97. doi: 10.1021/am5007029. Epub 2014 May 22.

DNA molecular beacon-based plastic biochip: a versatile and sensitive scanometric detection platform.

Author information

1
Department of Chemistry, Beijing Normal University , Beijing 100875, P. R. China.

Abstract

In this paper, we report a novel DNA molecular beacon (MB)-based plastic biochip platform for scanometric detection of a range of analytical targets. Hairpin DNA strands, which are dually modified with amino and biotin groups at their two ends are immobilized on a disposable plastic (polycarbonate) substrate as recognition element and gold nanoparticle-assisted silver-staining as signal reading protocol. Initially, the immobilized DNA probes are in their folded forms; upon target binding the hairpin secondary structure of the probe strand is "forced" open (i.e., converted to the unfolded state). Nanogold-streptavidin conjugates can then bind the terminal biotin groups and promote the deposition of rather large silver particles which can be either directly visualized or quantified with a standard flatbed scanner. We demonstrate that with properly designed probe sequences and optimized preparation conditions, a range of molecular targets, such as DNA strands, proteins (thrombin) and heavy metal ions (Hg(2+)), can be detected with high sensitivity and excellent selectivity. The detection can be done in both standard physiological buffers and real world samples. This constitutes a platform technology for performing rapid, sensitive, cost-effective, and point-of-care (POC) chemical analysis and medical diagnosis.

KEYWORDS:

DNA molecular beacon; medical diagnosis; plastic biochip; scanometric detection; target-induced conformation switching

PMID:
24852130
DOI:
10.1021/am5007029
[Indexed for MEDLINE]

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