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J Antibiot (Tokyo). 2014 Nov;67(11):755-61. doi: 10.1038/ja.2014.65. Epub 2014 May 21.

Combination cellulose plate (non-agar solid support) and agar plate method improves isolation of fungi from soil.

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1] Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan [2] Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.
Kyokuto Pharmaceutical Industrial Co., Ltd., Tokyo, Japan.
Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.


This is the first report describing the improved isolation of common filamentous fungi via a method combining cellulose plate and agar plate system. A cellulose plate is a porous plate made of nanofibrous crystaline cellulose. Isolating fungi from soils using these types of media separately resulted in the number of fungal colonies appearing on cellulose plates being lower than that on agar plates. However, the number of actual fungal species isolated using cellulose plates alone was more or less the same as that found using agar plates. Significantly, the diversity of isolates using a combination of the two media was greater than using each media individually. As a result, numerous new or rare fungal species with potential, including previously proposed new species, were isolated successfully in this way. All fungal colonies, including the Penicillium species, that appeared on the cellulose plate penetrated in potato dextrose were either white or yellow. Cultivation on cellulose plates with added copper ion overcomes the change in coloration, the colonies appearing as they do following cultivation on potato dextrose agar.

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