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Nan Fang Yi Ke Da Xue Xue Bao. 2014 May;34(5):736-9.

[Effect of emodin on rat bone marrow mesenchymal stem cell proliferation and mRNA expressions of hematopoietic growth factors].

[Article in Chinese]

Author information

1
Second College of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510120, China. E-mail: liuming3134@126.com.

Abstract

OBJECTIVE:

To study the effect of emodin on the proliferation, cell cycle distribution, apoptosis and expression of hematopoietic growth factors in bone marrow mesenchymal stem cells (BMSCs).

METHODS:

The proliferation of rat BMSCs exposed to emodin was analyzed using MTT assay, and flow cytometry was used to detect the apoptosis and cell cycle changes of the exposed cells. Real-time quantitative PCR was used to determine the mRNA expression of the hematopoietic growth factors.

RESULTS:

Exposure to 0.1 and 1 µg/ml emodin for 48 and 72 h significantly enhanced the proliferation of BMSCs (P<0.01). The cells exposed to 0.1 µg/ml emodin showed significantly increased percentage of cells in G2/M phase (P<0.05), and 1 µg/ml emodin exposure caused increased cells in S phase (P<0.01) and decreased cells in G1/G0 phase (P<0.05). Emodin exposure for 48 h resulted in significantly decreased cell apoptosis (P<0.05). BMSCs treated with 0.1 µg/ml emodin showed a significant increase in the expression of thrombopoietin mRNA (P<0.05).

CONCLUSION:

Emodin can promote the proliferation of BMSCs in vitro possibly by regulating the cell cycle distribution, cell apoptosis and thrombopoietin expression.

PMID:
24849448
[Indexed for MEDLINE]
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