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Can J Microbiol. 2014 Jun;60(6):355-61. doi: 10.1139/cjm-2014-0178. Epub 2014 Apr 17.

Quorum-sensing gene luxS regulates flagella expression and Shiga-like toxin production in F18ab Escherichia coli.

Author information

1
a College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, People's Republic of China.

Abstract

To investigate the effect of the luxS gene on the expression of virulence factors in Shiga-like toxin producing and verotoxin-producing Escherichia coli, the luxS gene from E. coli 107/86 (wild type, O139:H1:F18ab, Stx2e) was deleted. The successful deletion of luxS was confirmed by bioluminescence assays. The luxS deletion mutant exhibited changed flagella-related phenotypes, like impaired expression of flagella, decreased flagella motility, reduced biofilm formation, and reduced ability to induce pro-immunity response in host cells, which were restored after complementation with the intact luxS gene. The mutant strain also displayed attenuated production of Stx2e. This study provides new information to the crucial function of luxS in regulating Shiga-like toxin producing E. coli virulence.

KEYWORDS:

Escherichia coli; d├ętection du quorum; flagella; flagelles; luxS; quorum sensing

PMID:
24848979
DOI:
10.1139/cjm-2014-0178
[Indexed for MEDLINE]

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