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FASEB J. 2014 Sep;28(9):4169-79. doi: 10.1096/fj.14-250167. Epub 2014 May 20.

Metabolome and fecal microbiota in monozygotic twin pairs discordant for weight: a Big Mac challenge.

Author information

1
VTT Technical Research Centre of Finland, Espoo, Finland; Department of Food Science and Physiology, University of Navarra, Pamplona, Spain;
2
VTT Technical Research Centre of Finland, Espoo, Finland;
3
Obesity Research Unit, Research Programs Unit, Diabetes and Obesity, Department of Psychiatry.
4
Department of Public Health, Hjelt Institute, and Institute for Molecular Medicine Finland (FIMM), University of Helsinki, Helsinki, Finland; National Institute for Health and Welfare, Helsinki, Finland; and.
5
Department of Medicine, Division of Endocrinology, Helsinki University Central Hospital, Helsinki, Finland;
6
Department of Radiology, The Hospital District of Helsinki and Uusimaa (HUS) Medical Imaging Center, and.
7
VTT Technical Research Centre of Finland, Espoo, Finland; Steno Diabetes Center, Gentofte, Denmark.
8
Obesity Research Unit, Research Programs Unit, Diabetes and Obesity, Institute for Molecular Medicine Finland (FIMM), University of Helsinki, Helsinki, Finland; Department of Medicine, Division of Endocrinology, Helsinki University Central Hospital, Helsinki, Finland;
9
VTT Technical Research Centre of Finland, Espoo, Finland; Steno Diabetes Center, Gentofte, Denmark mtjo@steno.dk.

Abstract

Postprandial responses to food are complex, involving both genetic and environmental factors. We studied postprandial responses to a Big Mac meal challenge in monozygotic co-twins highly discordant for body weight. This unique design allows assessment of the contribution of obesity, independent of genetic liability. Comprehensive metabolic profiling using 3 analytical platforms was applied to fasting and postprandial serum samples from 16 healthy monozygotic twin pairs discordant for weight (body mass index difference >3 kg/m(2)). Nine concordant monozygotic pairs were examined as control pairs. Fecal samples were analyzed to assess diversity of the major bacterial groups by using 5 different validated bacterial group specific denaturing gradient gel electrophoresis methods. No differences in fecal bacterial diversity were detected when comparing co-twins discordant for weight (ANOVA, P<0.05). We found that within-pair similarity is a dominant factor in the metabolic postprandial response, independent of acquired obesity. Branched chain amino acids were increased in heavier as compared with leaner co-twins in the fasting state, but their levels converged postprandially (paired t tests, FDR q<0.05). We also found that specific bacterial groups were associated with postprandial changes of specific metabolites. Our findings underline important roles of genetic and early life factors in the regulation of postprandial metabolite levels.

KEYWORDS:

bile acids; metabolomics; obesity

PMID:
24846387
PMCID:
PMC5395738
DOI:
10.1096/fj.14-250167
[Indexed for MEDLINE]
Free PMC Article

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