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FEBS Lett. 2014 Jun 13;588(13):2170-6. doi: 10.1016/j.febslet.2014.05.011. Epub 2014 May 17.

Induction of miR-29a by saturated fatty acids impairs insulin signaling and glucose uptake through translational repression of IRS-1 in myocytes.

Author information

1
Department of Biochemistry, Dongguk University College of Medicine, Gyeongju 780-714, Republic of Korea.
2
Department of Biochemistry, Dongguk University College of Medicine, Gyeongju 780-714, Republic of Korea; Endocrine Channelopathy, Channelopathy Research Center, Dongguk University College of Medicine, Goyang 410-773, Republic of Korea. Electronic address: wanlee@dongguk.ac.kr.

Abstract

MicroRNAs have been shown to play an important role in insulin signaling but their biological function in insulin resistance induced by saturated fatty acids (SFA) remains largely unknown. Here, we report that SFA palmitate and high fat diet (HFD) significantly increase expression of miR-29a in myocytes. miR-29a targets IRS-1 3'UTR directly and represses IRS-1 expression at the translational level. Furthermore, the ectopic expression of miR-29a impairs insulin signaling and glucose uptake in myocytes through a substantial decrease in IRS-1. These findings suggest that the up-regulation of miR-29a by SFA is causally related to the development of insulin resistance in myocytes.

KEYWORDS:

IRS-1; Insulin resistance; MicroRNA; Palmitate; Saturated fatty acid; miR-29a

PMID:
24844433
DOI:
10.1016/j.febslet.2014.05.011
[Indexed for MEDLINE]
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