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Am J Physiol Lung Cell Mol Physiol. 2014 Jul 15;307(2):L149-57. doi: 10.1152/ajplung.00396.2012. Epub 2014 May 16.

Nicotine alters mucin rheological properties.

Author information

1
Bioengineering, University of California at Merced, Merced, California; Center for Biomedical Engineering, Chang Gung University, Taoyuan, Taiwan; and MicroBase Technology Corporation, Taoyuan, Taiwan.
2
Bioengineering, University of California at Merced, Merced, California;
3
Bioengineering, University of California at Merced, Merced, California; wchin2@ucmerced.edu.

Abstract

Tobacco smoke exposure, the major cause of chronic obstructive pulmonary disease (COPD), instigates a dysfunctional clearance of thick obstructive mucus. However, the mechanism underlying the formation of abnormally viscous mucus remains elusive. We investigated whether nicotine can directly alter the rheological properties of mucin by examining its physicochemical interactions with human airway mucin gels secreted from A549 lung epithelial cells. Swelling kinetics and multiple particle tracking were utilized to assess mucin gel viscosity change when exposed to nicotine. Herein we show that nicotine (≤50 nM) significantly hindered postexocytotic swelling and hydration of released mucins, leading to higher viscosity, possibly by electrostatic and hydrophobic interactions. Moreover, the close association of nicotine and mucins allows airway mucus to function as a reservoir for prolonged nicotine release, leading to correlated pathogenic effects. Our results provide a novel explanation for the maltransport of poorly hydrated mucus in smokers. More importantly, this study further indicates that even low-concentration nicotine can profoundly increase mucus viscosity and thus highlights the health risks of secondhand smoke exposure.

KEYWORDS:

chronic obstructive pulmonary disease; environmental tobacco smoke; mucin swelling kinetics; mucociliary clearance; tobacco smoke; viscosity

PMID:
24838753
PMCID:
PMC4101795
DOI:
10.1152/ajplung.00396.2012
[Indexed for MEDLINE]
Free PMC Article
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