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Food Chem. 2014 Oct 15;161:60-6. doi: 10.1016/j.foodchem.2014.03.119. Epub 2014 Apr 2.

Influence of molecular weight on in vitro immunostimulatory properties of instant coffee.

Author information

1
QOPNA, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal. Electronic address: cpassos@ua.pt.
2
QOPNA, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
3
UTAD, Department of Chemistry, University of Trás-os-Montes e Alto Douro, 5001-801 Vila Real, Portugal.
4
CICECO, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
5
ICBAS, Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, 4050-313 Porto, Portugal; IBMC, Instituto de Biologia Molecular e Celular, Universidade do Porto, 4150-180 Porto, Portugal.

Abstract

Instant coffee was prepared and fractionated into higher (>100kDa), medium (5-10, 10-30, 30-100kDa) and lower (1-5, <1kDa) molecular weight fractions. Sugars and linkage composition characteristics of arabinogalactans and galactomannans were recovered in all fractions. Also, amino acid analysis performed after hydrolysis showed similar compositions in all fractions. On the contrary, free chlorogenic acids and caffeine were only detected in the lowest molecular weight fraction (<1kDa). A direct relationship between the melanoidins browning index and the molecular weight was observed. The fractions obtained were incubated in vitro with murine spleen lymphocytes in order to evaluate their possible immunostimulatory abilities. The surface expression of CD69 (early activation marker) on different lymphocyte sub-populations showed that the fraction with 1-5kDa was able to induce activation of B-lymphocytes. This was the only fraction to induce B-lymphocyte activation, since all the other fractions failed, even when higher concentrations were used.

KEYWORDS:

Caffeine; Chlorogenic acids; Immunostimulatory activity; Melanoidins; Polysaccharides; Ultrafiltration

PMID:
24837922
DOI:
10.1016/j.foodchem.2014.03.119
[Indexed for MEDLINE]

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