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Nat Methods. 2014 Jul;11(7):727-730. doi: 10.1038/nmeth.2964. Epub 2014 May 18.

Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy.

Author information

1
Research Institute of Molecular Pathology, Vienna, Austria.
2
Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.
3
Research Platform Quantum Phenomena & Nanoscale Biological Systems (QuNaBioS), University of Vienna, Austria.
4
Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.
5
MIT Media Lab, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.
6
Department of Mechanical Engineering, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.
7
Department of Biological Engineering, Massachusetts Institute of Technology(MIT), Cambridge, MA, USA.
8
Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.
9
McGovern Institute, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.
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Contributed equally

Abstract

High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique captures the dynamics of spiking neurons in volumes of ∼700 μm × 700 μm × 200 μm at 20 Hz. Its simplicity makes it an attractive tool for high-speed volumetric calcium imaging.

PMID:
24836920
PMCID:
PMC4100252
DOI:
10.1038/nmeth.2964
[Indexed for MEDLINE]
Free PMC Article

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