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Cytotherapy. 2014 Aug;16(8):1117-20. doi: 10.1016/j.jcyt.2014.02.004. Epub 2014 May 13.

Simplified protocol for clinical-grade tumor-infiltrating lymphocyte manufacturing with use of the Wave bioreactor.

Author information

1
Center for Cancer Immune Therapy, Department of Hematology, Herlev, Denmark; Department of Oncology, Herlev Hospital, Herlev, Denmark; Department of Bio-medical Sciences, University of Catania, Italy. Electronic address: marco.donia@regionh.dk.
2
Center for Cancer Immune Therapy, Department of Hematology, Herlev, Denmark.
3
Center for Cancer Immune Therapy, Department of Hematology, Herlev, Denmark; Department of Oncology, Herlev Hospital, Herlev, Denmark.
4
Center for Cancer Immune Therapy, Department of Hematology, Herlev, Denmark; Department of Oncology, Herlev Hospital, Herlev, Denmark. Electronic address: inge.marie.svane@regionh.dk.

Abstract

BACKGROUND AIMS:

The high level of complexity of current Good Manufacturing Practice-compliant methods of manufacturing hampers rapid and broad application of treatment with tumor-infiltrating lymphocytes (TILs).

METHODS:

To ensure higher applicability of TIL production to laboratory routine, a practical and simple protocol of TIL manufacturing with the use of a closed-system bioreactor was developed and implemented at our institution.

RESULTS:

This protocol enabled significant work load reduction during the most labor-intense step of TIL expansion, and allowed generation of high-quality TIL products, which mediated clinical regression in patients with metastatic melanoma.

CONCLUSIONS:

Implementation of simplified methods of TIL expansion will speed up dissemination of TIL methods worldwide and will increase patient access to this highly effective treatment.

KEYWORDS:

Wave bioreactor; metastatic melanoma; rapid expansion; tumor-infiltrating lymphocytes

PMID:
24831841
DOI:
10.1016/j.jcyt.2014.02.004
[Indexed for MEDLINE]

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