Format

Send to

Choose Destination
Nucleic Acids Res. 2014 Jul;42(Web Server issue):W205-9. doi: 10.1093/nar/gku393. Epub 2014 May 14.

PrecisePrimer: an easy-to-use web server for designing PCR primers for DNA library cloning and DNA shuffling.

Author information

1
Institute of System and Synthetic Biology, Université d'Évry val d'Éssonnes, Bt. Geneavenir 6 Genopole Campus 1, 5 rue Henry Desbruères 91000, Évry, France.
2
Institute of System and Synthetic Biology, Université d'Évry val d'Éssonnes, Bt. Geneavenir 6 Genopole Campus 1, 5 rue Henry Desbruères 91000, Évry, France jean-loup.faulon@issb.genopole.fr.

Abstract

PrecisePrimer is a web-based primer design software made to assist experimentalists in any repetitive primer design task such as preparing, cloning and shuffling DNA libraries. Unlike other popular primer design tools, it is conceived to generate primer libraries with popular PCR polymerase buffers proposed as pre-set options. PrecisePrimer is also meant to design primers in batches, such as for DNA libraries creation of DNA shuffling experiments and to have the simplest interface possible. It integrates the most up-to-date melting temperature algorithms validated with experimental data, and cross validated with other computational tools. We generated a library of primers for the extraction and cloning of 61 genes from yeast DNA genomic extract using default parameters. All primer pairs efficiently amplified their target without any optimization of the PCR conditions.

PMID:
24829457
PMCID:
PMC4086104
DOI:
10.1093/nar/gku393
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center