Format

Send to

Choose Destination
Chembiochem. 2014 Jun 16;15(9):1257-62. doi: 10.1002/cbic.201402142. Epub 2014 May 14.

Designed di-heme binding helical transmembrane protein.

Author information

1
School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore-637551 (Singapore).

Erratum in

  • Chembiochem. 2014 Jun 16;15(9):1221.

Abstract

De novo designing of functional membrane proteins is fundamental in terms of understanding the structure, folding, and stability of membrane proteins. In this work, we report the design and characterization of a transmembrane protein, termed HETPRO (HEme-binding Transmembrane PROtein), that binds two molecules of heme in a membrane and catalyzes oxidation/reduction reactions. The primary structure of HETPRO has been optimized in a guided fashion, from an antimicrobial peptide, for transmembrane orientation, defined 3D structure, and functions. HETPRO assembles into a tetrameric form, from an apo dimeric helical structure, in complex with cofactor in detergent micelles. The NMR structure of the apo HETPRO in micelles reveals an antiparallel helical dimer that inserts into the nonpolar core of detergent micelles. The well-defined structure of HETPRO and its ability to bind to heme moieties could be utilized to develop a functional membrane protein mimic for electron transport and photosystems.

KEYWORDS:

NMR spectroscopy; heme binding; membrane proteins; peptidomimetics; peroxidases

PMID:
24829076
DOI:
10.1002/cbic.201402142
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center